CatalogNo.:2423

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Caspase-8(FLICE)and-10(FLICE2)aretwopivotalmembersoftheICE/CED-3proteasefamily.FLICE-inhibitoryproteinshavebeenidentifiedinvirusesandhumancellsandaredesignatedv-FLIPsandFLIP,respectively.

HumanFLIPwasclonedbyseveralindependentlaboratoriesanddesignatedCasper,I-FLICE,FLAME-1,CASH,andCLARP.FLIPcontainstwodeatheffectordomainsandacaspase-likedomain.FLIPinteractswithadapterproteinFADDandcaspase-8and-10andpotentlyinhibitsapoptosisinducedbydeathreceptorsCD95,DR3,TRAIL-R,andTNFR1.FoursplicevariantsofFLIPhavebeenidentifiedanddesignatedFLIP,,,and.

Antigen:Peptidecorrespondingtoaa449-465attheC-terminusofmouseFLIPL/CASHform(accessionno.NP_997536).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizesmouseFLIPLform(55kDa)butnotFLIPSform.

Immunoblotting:useat2ug/ml.

WesternblotanalysisofmFLIPinNIH/3T3wholecelllysatewithanti-mFLIP(CT)at2ug/ml.

Positivecontrol:WholecelllysatefromNIH/3T3cells.

Immunocytochemistry:useat5ug/ml.

ImmunocytochemicalstainingofNIH/3T3cellswithmFLIPantibodyat5ug/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.