Anti-FLIP(NT)Antibody
CatalogNo.:1159
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Caspase-8(FLICE)and-10(FLICE2)aretwopivotalmembersoftheICE/CED-3proteasefamily.FLICE-inhibitoryproteinshavebeenidentifiedinvirusesandhumancellsandaredesignatedv-FLIPsandFLIP,respectively.HumanFLIPwasclonedbyseveralindependentlaboratoriesanddesignatedCasper,I-FLICE,FLAME-1,CASH,andCLARP.FLIPcontainstwodeatheffectordomainsandacaspase-likedomain.FLIPinteractswithadapterproteinFADDandcaspase-8and-10andpotentlyinhibitsapoptosisinducedbyallknowndeathreceptors.FoursplicevariantsofFLIPshavebeenidentifiedanddesignatedFLIP,,,and.
Antigen:Peptidecorrespondingtoaa2-18ofhumanFLIP(accessionno.AAC51622).ThissequenceisidenticalinallFLIPsplicevariants.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesallhuman,mouse,andratFLIPsplicevariants:,,,and.
APPLICATIONSImmunoblotting:useat1-2ug/ml
WesternblotanalysisofFLIPinK562celllysatewithFLIPantibodyat(A)1and(B)2μg/ml.
Positivecontrol:K562celllysate.Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofFLIPinHeLacellswithFLIPantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
