Anti-FLIPγ/δ(CT)Antibody
CatalogNo.:2407
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Caspase-8(FLICE)and-10(FLICE2)aretwopivotalmembersoftheICE/CED-3proteasefamily.FLICE-inhibitoryproteinshavebeenidentifiedinvirusesandhumancellsandaredesignatedv-FLIPsandFLIP,respectively.
HumanFLIPwasclonedbyseveralindependentlaboratoriesanddesignatedCasper,I-FLICE,FLAME-1,CASH,andCLARP.FLIPcontainstwodeatheffectordomainsandacaspase-likedomain.FLIPinteractswithadapterproteinFADDandcaspase-8and-10andpotentlyinhibitsapoptosisinducedbydeathreceptorsCD95,DR3,TRAIL-R,andTNFR1.FoursplicevariantsofFLIPhavebeenidentifiedanddesignatedFLIP,,,and.
Antigen:Peptidecorrespondingtoaa191-209attheC-terminusofhumanFLIP/FLIPSform(accessionno.AAC51623).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman(35kDa)andFLIP(25kDa)butnotFLIPorFLIP.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofFLIPincelllysatesfromHeLa(H),Jurkat(J),THP-1(T),A431(A),K562(K)andNIH3T3
(N)cellswithFLIPγ/δantibodyat1ug/ml.
Positivecontrol:WholecelllysatefromHeLa,Jurkat,THP-1,A431,K562,orNIH3T3cells.
Immunocytochemistry:useat2ug/ml.
ImmunocytochemicalstainingofHeLacellsusingFLIPγ/δantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
