Anti-ICAD(CT)Antibody
CatalogNo.:2424
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AhumanDNAfragmentationfactor(DFF)thatiscleavedbycaspase-3duringapoptosishasbeenidentified.ThemousehomologueofhumanDFFwasidentifiedasaDNaseinhibitorandwasdesignatedICAD(inhibitorofcaspase-activatedDNase).
UponcleavageofDFF/ICAD,acaspaseactivateddeoxyribonuclease(CAD)isreleasedandactivatedandeventuallycausesthedegradationofDNAinnuclei.Therefore,cleavageofCADinhibitormoleculeDFF/ICAD,whichcausesDNaseactivationandDNAdegradation,isahallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa312-331attheC-terminusofmouseICAD(accessionno.O54786).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesnon-cleaved(45kDa)andcleavedmouseICAD.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofICADinmurinelung(L),brain(B),liver(L),andkidneytissuelysatewithanti-ICAD(CT)at1ug/ml.
Positivecontrol:Mouselungtissuelysate.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
