Anti-ICAD(NT)Antibody
CatalogNo.:2425
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AhumanDNAfragmentationfactor(DFF)thatiscleavedbycaspase-3duringapoptosiswasidentifiedrecently.ThemousehomologueofhumanDFFwasidentifiedasaDNaseinhibitorandwasdesignatedICAD(inhibitorofcaspase-activatedDNase).UponcleavageofDFF/ICAD,acaspaseactivateddeoxyribonuclease(CAD)isreleasedandactivatedandeventuallycausesthedegradationofDNAinnuclei.Therefore,cleavageofCADinhibitormoleculeDFF/ICAD,whichcausesDNaseactivationandDNAdegradation,isahallmarkofapoptoticcelldeath.
Antigen:Peptidecorrespondingtoaa2-21attheN-terminusofmouseICAD(accessionno.O54786).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesnon-cleaved(45kDa)andcleavedmouseICAD.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofICADinmurinelungtissuelysatewithICADantibodyat1μg/ml.
Positivecontrol:Mouselungtissuelysate.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofICADinmurinelungtissuewithICADantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
