CatalogNo.:11005

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

ApoptosisistriggeredbyavarietyofstimuliincludingmembersintheTNFfamilyandpreventedbytheinhibitorofapoptosis(IAP)proteins.IAPproteinsformaconservedgenefamilyincludingIAP,XIAP/ILP-1/MIHA,andLivin/KIAPthatbindtoandinhibitspecificproteases.AnovelmemberintheIAPproteinfamilyhasbeenidentifiedanddesignatedILP-2forIAP-likeprotein-2(1).

ILP-2hashighhomologytoILP-1,butisencodedbyadistinctgene.ILP-2,unlikeILP-1,hasnoinhibitoryeffectonFasandTNFinducedapoptosis,butpotentlyinhibitsapoptosisinducedbyoverexpressionofBax

orbycoexpressionofcaspase-9withApaf-1.ILP-2interactswiththeprocessedcaspase-9.

Antigen:Peptidecorrespondingtoaa2-13ofhumanILP-2(accessionno.NP_203127).HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

Thisantibodyrecognizeshuman,mouse,andratILP-2(33kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofILP-2inhumanHepG2(lane

1)andMOLT4(lane2)celllysateswithILP-2antibodyat1μg/ml.

Positivecontrol:HepG2orMOLT4celllysates.

Immunocytochemistry:useat10ug/ml.

ImmunocytochemicalstainingofILP-2inHepG2cellswithILP-2antibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.