Anti-LivinAntibody
CatalogNo.:11006
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Apoptosisispreventedbytheinhibitorofapoptosis(IAP)proteins.AnovelmemberintheIAPproteinfamilyhasbeenidentifiedanddesignatedLivinandKIAPforkidneyIAP.Livin/KIAPcontainsasinglebaculoviralIAPrepeat(BIR)domainandaRINGfingerdomainandhastwoisoformstermedLivin-aandLivin-b.TransfectionofLivinincellsresultsinprotectionfromapoptosisinducedbyFADD,BAX,RIP,RIP3andDR6.Livin
hasdirectinteractionwithseveralcaspasesincludingcaspase-3,-7,and-9.Livininhibitstheactivationofcaspase-9inducedbyApaf-1,cytochromec,anddATP.ThetwoisoformsofLivinappeartohavedifferentfunctionsandtissuedistributions.
Antigen:Peptidecorrespondingtoaa264-280oftheshortformandaa281-298ofthelongformofhumanLivin(accessionno.
NP_071444).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThisantibodyrecognizeshumanLivin(33kDa).
APPLICATIONSImmunoblotting:useat0.5-1.0ug/ml.
WesternblotanalysisofLivinexpressioninhumanRajicelllysatewithLivinantibodyat0.5μg/ml.
Positivecontrol:Rajicelllysate.
Immunohistochemistry:useat5ug/ml.
ImmunohistochemicalstainingofLivininhumansmallintestinewithLivinantibodyat5μg/ml.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
