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CatalogNo.:1150

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.

MAPkinase-activatingdeathdomainprotein(MADD)wasinitiallyidentifiedasthetype1tumornecrosisfactorreceptor(TNFR1).

OverexpressionofMADDactivatesMAPkinasesERKandJNKandinducesthephosphorylationofcytosolicphospholipaseA2.MADDshares98%homologywithDENN(differentiallyexpressedinneoplasicvs.normalcells)whichwasrecentlyidentifiedasasubstrateforc-junN-terminalkinase3(JNK3).MADDhasgreatherthan94%homologywithaGDO/GTPexchangeprotein,Rab3-GEP,anditis87%homologouswithKIAA0358,abrainproteinofunknownfunction.IdentificationofMADDasacomponentoftheTNFR1signallingcomplexandthesimilaritybetweenMADDandRab3-GEPprovidesaconnectionbetweenTNFR1activationanddownstreamMAPkinaseactivitythroughaguanine-nucleotideexchangeprotein.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Antigen:Peptidecorrespondingtoaa1570-1588ofhumanMADD.ThispeptidesequenceisidenticaltothatofDENNanddiffersbyoneaminoacidwithratGDP/GTPexchangeproteinRAB3-GEP.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanandmouseMADD(200-220kD).

Immunoblotting:useat1:250-1:500dilution.

Positivecontrol:WholecelllysatefromHeLaorNIH3T3cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

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