Anti-NFκB,p50Antibody
CatalogNo.:12508-2525ug
12508-100100ug
Size:RabbitIgGinborate-bufferedsaline,pH8.2,purifiedbyimmunoaffinitychromatography.
NuclearfactorkappaB(NFkappaB)wasidentifiedasasequencespecifictranscriptionalactivatorthatbindstotheintronicenhancerofthekappalightchaingeneinBlymphocytes.NFkappaBisaheterodimerthatconsistsofa50kDaDNAbindingsubunit(p50)anda65kDatransactivationsubunit(p65/RelA).Thep50/p65heterodimerremainsinthecytosolinaninactiveformasacomplexwithitsinhibitor,IkappaB.Uponstimulationofcellsbyawidevarietyofstimulisuchaslipopolysaccharide(LPS),pro-inflammatorycytokines,andviralinfection,NFkappaBisphosphorylatedanddegradedbytheproteosome.TheactiveNFkappaBheterodimeristranslocatedintothenucleusandinducesgeneexpression.
Immunogen:Syntheticpeptidecorrespondingtoaa4-21ofmouseNFκBp50.
HostSpecies:Rabbit
StABIlizers:50%glycerol
Preservatives:0.09%sodiumazide
ThisantibodyrecognizesmouseandratNF-κBp50.Detectsabandof~50kDainWesternblot.
APPLICATIONSImmunoblotting:useat0.5-1.0ug/ml.
WesternblotanalysisofNFκBinratspleentissuelysatewithNFκBantibodyat0.5ug/ml.
Positivecontrol:Ratspleentissuelysate.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
