Anti-PHAP(CT)Antibody
CatalogNo.:11090
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.
Antigen:SyntheticpeptidecorrespondingtoaminoacidsnearthecarboxyterminusofhumanPHAP(accessionno.P39687).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
Thisantibodyrecognizeshuman,mouseandratPHAPI(32kDa)andPHAPIII(35kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSWesternBlot:Useat1ug/ml.
WesternblotanalysisofPHAPexpressioninRajicelllysatewithPHAPantibodyat1μg/ml.ThetwobandsbelowPHAPImightbedifferentlysplicedisoformsofPHAP.
Positivecontrol:Rajicelllysate
Immunohistochemistry:useat10ug/ml.
ImmunohistochemicalstainingofPHAPinhumansmallintestinewithPHAPantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
