Anti-PHAP(NT)Antibody
CatalogNo.:11086
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:SyntheticpeptidecorrespondingtoaminoacidsneartheaminoterminusofhumanPHAP(accessionno.P39687).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.
Thisantibodyrecognizesallthreeisoformsofhuman,mouseandratPHAP,includingPHAPI(32kDa),PHAPI2a,andPHAPIII(35kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:Useat1ug/ml.
WesternblotanalysisofPHAPinRajicelllysatewithPHAPantibodyat1μg/ml.ThewideandstrongbandbelowPHAPIIIisadoubletcomposedofPHAPI(upper)andPHAPI2a(lower).
Positivecontrol:Rajicelllysate.
Immunocytochemistry:useat1ug/ml.
ImmunocytochemicalstainingofPHAPinRajicellswithPHAPantibodyat1μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
