Anti-PHAPI(CT)Antibody
CatalogNo.:11088
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:AsyntheticpeptidecorrespondingtoaminoacidsatthecarboxyterminusofhumanPHAP(accessionno.P39687).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.
Thisantibodyrecognizeshuman,mouseandratPHAPI(32kDa),anddoesnotcross-reactwithPHAPI2aorPHAPIII.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:Useat1ug/ml.
WesternblotanalysisofPHAPIexpressioninhumanRajicell(A),mouse(B)andrat(C)testistissuelysateswithPHAPIantibodyat1μg/ml.
Positivecontrol:Rajicelllysate.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofPHAPIinmousesmallintestinewithPHAPIantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
