Anti-PHAPI(IN)Antibody
CatalogNo.:11082
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Antigen:SyntheticpeptidecorrespondingtoaminoacidsnearthecarboxyterminusofhumanPHAPI(accessionno.P39687).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide
ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.
ThisantibodyrecognizeshumanandmousePHAPI(32kDa).ThesequenceofratPHAPIisidenticaltohumanPHAPI.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:Useat2-4ug/ml.
WesternblotanalysisofPHAPIinRajicelllysatewithPHAPIantibodyat2μg/ml(laneA)and4μg/ml(laneB).
Positivecontrol:Rajicelllysate.
Immunocytochemistry:useat2ug/ml.
ImmunocytochemicalstainingofPHAPIinRajicellswithPHAPIantibodyat2ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
