CatalogNo.:11084

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Antigen:SyntheticpeptidecorrespondingtoaminoacidsnearthecarboxyterminusofhumanPHAPIII(accessionno.NP_112182)HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.

Thisantibodyrecognizeshuman,mouseandratPHAPIII(35kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

WesternBlot:Useat1ug/ml.

WesternblotanalysisofPHAPIIIinA549(A)andHepG2(B)cells,andrattestis(C)withPHAPIIIantibodyat1μg/ml.

Positivecontrol:A549celllysate.

Immunohistochemistry:useat2ug/ml.

ImmunohistochemicalstainingofPHAPIIIinhumansmallintestinewithPHAPIIIantibodyat2μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.