CatalogNo.:11008

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

PUMA(p53UpregulatedModulatorofApoptosis)isapro-apoptoticBcl-2familymemberthatencodestwoBH3domain-containingproteins,PUMA-andPUMA-.PUMAproteinsbindBcl-2,localizetomitochondria,andinducecytochromecreleaseandapoptosisinresponsetop53.

Antigen:Syntheticpeptidecorresponding

toaa2-16ofhumanPUMA-(accessionno.NP_055232)..ThissequenceisidenticalinmousePUMA-.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

ThisantibodyrecognizeshumanandmousePUMA(23kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat2ug/ml.

WesternblotanalysisofPUMAinK562celllysatewithPUMAantibodyat2μg/ml.

Positivecontrol:K562celllysate.

Immunohistochemistry:useat10ug/ml.

ImmunohistochemicalstainingofPUMAinhumanbreastcarcinomawithPUMAantibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.