Anti-RAIDD(IN)Antibody
CatalogNo.:1115
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelcelldeathadapterwasrecentlyidentifiedbytwoindependentlaboratoriesanddesignatedRAIDD(RIP-associatedICH-1/CED-3-homologousproteinwithDD)andCRADD(caspaseandRIPadapterwithDD).RAIDDcontainsaDDandaCARD(caspaserecruitmentdomain)whichinteractwithRIPandcaspase,respectively,totransducedeathsignals.RAIDDis
constitutivelyexpressedinmanytissuesandmediatesapoptosiscausedbyFasandTNFR1.
Antigen:Peptidecorrespondingtoaa99-117ofhumanRAIDD(accessionno.
AAB42217).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanRAIDD(22kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofRAIDDinwholecelllysatesfromHeLa(H)orK562(K)cellswithRAIDDantibodyat
1g/ml.
Positivecontrol:HeLaorK562celllysate.
Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofRAIDDinHeLacellswithRAIDDantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
