Anti-RasAntibody
CatalogNumber | Quantity |
11068-50 | 50ug |
11068-200 | 200ug |
CatalogNumber | Quantity |
11068-50 | 50ug |
11068-200 | 200ug |
ORDERINGINFORMATION
MonomericG-proteinRasfunctionsasamolecularswitchlinkingreceptorandnonreceptortyrosinekinaseactivationtoanumberofdownstreamsignalingpathways.Oncogenicmutations,whichoccurinapproximately30%ofallcasesofhumancancer,leadtothestimulationofproliferationandtheinhibitionofapoptosis.
Antigen:A13-residuesyntheticpeptidefromtheeffectorbindingloopofH-,K-,andN-Ras,residues31-43(EYDPTIEDSYRKQ((C).
HostSpecies:Rabbit
Format:AffinityPurified
Buffer:PBS,pH7.2,50%glycerol,0.09%sodiumazide.
Thisantibodyrecognizeshuman,mouse,ratandbovineRas.Itdetectsabandof21-23kDabyWesternblot.
APPLICATIONSImmunoblotting:useat1–4ug/ml.
WesternblotanalysisofRas:Lane1:MWMarker,Lane2:HeLalysate,MouseBrainLysate,RatBrainLysate.
Positivecontrol:HeLacelllysate.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediawhichisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
