Anti-RICK(CT)Antibody
CatalogNo.:2183
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ACARD-containingserine/threoninekinasehasbeenidentifiedanddesignatedRICK/RIP2/CARDIAKforRIP-likeinteractingCLARPkinase,receptorinteractingprotein-2,andCARD-containingICEassociatedkinase,respectively.RICKcontainsanN-terminalkinasecatalyticdomainandaC-terminalCARDdomain.
OverexpressionofRICKinducesapoptosisandactivatesNF-BandJNK.RICKinteractswithmembersoftheTRAFfamily,CLARP,andcaspase-1.RICKregulatesTNFandFasinduced-apoptosisandisinvolvedinthegenerationoftheproinflammatorycytokineIL-1.ThemRNAofRICKisexpressedinmanyhumantissues.
Antigen:Syntheticpeptidecorrespondingtoaa508-522ofhumanRICK(accessionno.
AF027706).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratRICK(60kDa).
APPLICATIONSImmunoblotting:useat1-2ug/ml.
WesternblotanalysisofRICKinA431celllysatewithRICKantibodyat(A)1and(B)2μg/ml.
Positivecontrol:A431celllysate.
Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofRICKinK562cellswithRICKantibodyat5μg/ml.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
