Anti-RICK(NT)Antibody
CatalogNo.:2427
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ACARD-containingserine/threoninekinasethatregulatesapoptosishasbeenidentifiedanddesignatedRICK(RIP-likeinteractingCLARPkinase).RICKcontainsanN-terminalkinasecatalyticdomainandaC-terminalCARDdomain.RICKkinasedomainhashighsequencehomologytothatofRIP.OverexpressionofRICKpromotestheactivationofcaspase-8andFas-inducedapoptosis.RICKrepresentsa
novelkinasethatregulatesFasinduced-apoptosis.ThemessengerRNAofRICKisexpressedinavarietyofhumantissues.
Antigen:Syntheticpeptidecorrespondingtoaa11-30attheN-terminusofhumanRICK(accessionno.O43353).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizesfull-lengthhuman,mouse,andratRICK(60kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofRICKinA431(A)andK562(K)wholecelllysatewithRICKantibodyat1g/ml.
Positivecontrol:A431orK562celllysate.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofRICKinA431cellswithRICKantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
