CatalogNo.:2283

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Serine/threonineproteinkinases,suchasASK1,RIP,DAPandZIPkinases,aremediatorsofapoptosis.Receptorinteractingproteins,includingRIPandRIP2/RICKmediateapoptosisinducedbyTNFR1andFas,twomembersofthefamilyofdeathreceptors.AnovelmemberintheRIPkinasefamilyhasbeenidentifiedanddesignatedRIP3.RIP3containsanN-terminalkinasedomain,but,unlikeRIPorRIP2,lackstheC-terminaldeathorCARDdomain.RIP3bindstoRIPandTNFR1,mediatesTNFR1-inducedapoptosis,andattenuatesRIPandTNFR1-inducedNF-Bactivation.ThemessengerRNAofRIP3isfoundinsomeadulttissues.

Antigen:Syntheticpeptidecorrespondingtoaa473-486ofmouseRIP3(accessionno.

AAF03133).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizesmouseandratRIP3(57kDa).

Immunoblotting:useat1ug/ml.

WesternblotanalysisofRIP3inNIH/3T3wholecelllysateintheabsence(A)orpresence(B)ofblockingpeptidewithRIP3antibodyat1μg/ml.

Positivecontrol:NIH/3T3celllysate.

Immunohistochemistry:useat5ug/ml.

ImmunohistochemicalstainingofRIP3inratkidneywithRIP3antibodyat5μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.