Anti-Smac(CT)Antibody
CatalogNo.:1409
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Inhibitorsofapoptosisproteins(IAPs)regulateprogrammedcelldeathbyinhibitingmembersofthecaspasefamilyofenzymes.AnovelmammalianproteinthatbindstoIAPsandneutralizestheinhibitoryeffectofIAPsoncaspaseshasbeenidentifiedanddesignatedSmac/DIABLO.Smacisamitochondrialproteinthatisreleasedalongwithcytochromecduringapoptosisandactivatesthecytochromec/Apaf-1/caspase-9pathway.TheN-terminalaminoacidsofSmacarerequiredforbindingtoIAPsandforactivationofcaspases.Smacisexpressedinavarietyofhumanandmousetissues.
Antigen:Syntheticpeptidecorrespondingtoaa225-239ofhumanSmac(accessionno.
AAF87716).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratSmac(25kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofSmacinhumanhearttissuelysateintheabsence(A)orpresence(B)ofblockingpeptidewithSmacantibodyat1μg/ml.
Positivecontrol:Humanhearttissuelysate.
Immunohistochemistry:useat5ug/ml
ImmunohistochemicalstainingofSmacinhumanovarywithSmacantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
