Anti-Smac(mouseCT)Antibody
CatalogNo.:1411
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Inhibitorsofapoptosisproteins(IAPs)regulateprogrammedcelldeathbyinhibitingmembersofthecaspasefamilyofenzymes.AnovelmammalianproteinthatbindstoIAPsandneutralizestheinhibitoryeffectofIAPsoncaspaseshasbeenidentifiedanddesignatedSmac/DIABLO.Smacisamitochondrialproteinthatisreleasedalongwithcytochromecduringapoptosisandactivatesthecytochromec/Apaf-1/caspase-9pathway.TheN-terminalaminoacidsofSmacarerequiredforbindingtoIAPsandforactivationofcaspases.Smacisexpressedinavarietyofhumanandmousetissues.
Antigen:Syntheticpeptidecorrespondingtoaa222-237ofmouseSmac(accessionno.
AF203914).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratSmac(25kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofSmacinmousehearttissuelysateintheabsence(A)orpresence(B)of
blockingpeptideandinrathearttissuelysatewithmSmacantibodyat1μg/ml.
Positivecontrol:Mousehearttissuelysate.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofmousespleenusingmSmacantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
