Anti-SODD(NT)Antibody
CatalogNo.:2143
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
AnovelTNF-R1-interactingproteinhasbeenidentifiedanddesignatedSODD(silencerofdeathdomains).SODDassociateswiththedeathdomainofTNF-R1andpreventsconstitutiveactivationofTNF-R1signaling.TNFtreatmentreleasesSODDandpermitsadaptermoleculessuchasTRADDtoengageintheactiveTNF-R1complex,thusactivatingTNFsignalingpathways.SODDalsointeractswithDR3.
SODDisubiquitouslyexpressedinhumantissuesandcelllines.
Antigen:Syntheticpeptidecorrespondingtoaa2-16ofhumanSODD(accessionno.AF111116).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
Thisantibodyrecognizeshuman,mouse,andratSODD(60kDa).
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofSODDinHeLa(1,3)andTHP-1(2,4)wholecelllysatesintheabsence(1,2)orpresence(3,4)ofblockingpeptidewithSODDantibodyat1g/ml.
Positivecontrol:HeLaorTHP-1celllysate.
Immunocytochemistry:useat5ug/ml
ImmunocytochemicalstainingofSODDinHeLacellswithSODDantibodyat5μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
