Anti-Survivin(mouseCT)Antibody
CatalogNo.:2237
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Apoptosiscanbepreventedbytheinhibitorofapoptosisproteins(IAPs).IAPsconstituteaconservedgenefamilythatbindstoandinhibitscelldeathproteases.AnovelIAPhasbeenidentifiedanddesignatedSurvivin.Survivininteractswiththeprocessedformofcaspase-3andinhibitsitsproteolyticactivity.Survivinisexpressedpredominantlyintissuesofembryos,transformedcelllines,andmanyhumancancers.
Antigen:Syntheticpeptidecorrespondingtoaa128-140ofmouseSurvivin(accessionno.BAA28266).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizesmouseSurvivin(17kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofSurvivininmousespleentissuelysatewithSurvivinantibodyat1μg/ml.
Positivecontrol:Mousespleencelllysate.
Immunohistochemistry:useat10ug/ml.
ImmunohistochemicalstainingofSurvivininmousespleenwithSurvivinantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
