Anti-Survivin(NT)Antibody
CatalogNo.:2233
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
Apoptosiscanbepreventedbytheinhibitorofapoptosisproteins(IAPs).IAPsconstituteaconservedgenefamilythatbindstoandinhibitscelldeathproteases.AnovelIAPhasbeenidentifiedanddesignatedSurvivin.Survivininteractswiththeprocessedformofcaspase-3andinhibitsitsproteolyticactivity.Survivinisexpressedpredominantlyintissuesofembryos,transformedcelllines,andmanyhumancancers.
Antigen:Syntheticpeptidecorrespondingtoaa1-12ofhumanSurvivin(accessionno.
NP_001159).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanSurvivin(17kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofSurvivininMOLT4celllysatewithSurvivinantibodyat1μg/ml.
Positivecontrol:MOLT4celllysate.
Immunocytochemistry:useat5ug/ml.
ImmunocytochemicalstainingofSurvivininJurkatcellswithSurvivinantibodyat5ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
