CatalogNo.:1131

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

TNFissynthesizedasa26kDatypeIImembrane-boundprecursorthatiscleavedbyaconvertasetogeneratesecreted17kDamatureTNF.TNFconvertingenzyme(TACE)hasbeenidentified,andhumanandmouseTACECDNAshavebeencloned.TACEisamembrane-boundmetalloprotease-disintegrininthefamilyofmammalianADAM(foradisintegrinandmetalloprotease).TACEalsoprocessesothercellsurfaceproteins,includingTNFreceptor,TGF,L-selectin,andalpha-cleavageofamyloidproteinprecursor(APP).TACEmRNAisexpressedinavarietyofhumanandmousetissues.

Antigen:Peptidecorrespondingtoaa807-823ofhumanTACE(accessionno.NP_003174).

ThissequencediffersfrommouseandratTACEbyoneaminoacid.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizeshuman,mouse,andratTACE,and80-130kDabandsaredetectedinimmunoblots.Thesebandsmayrepresentmatureprotein,precursor,andglycosylatedTACE.

Immunoblotting:useat1ug/ml.

WesternblotanalysisofTACEinHeLa(1,3)andJurkat(2,4)celllysateintheabsence(1,2)orpresence(3,4)ofblockingpeptidewithTACEantibodyat1g/ml.

Positivecontrol:HeLaorJurkatcelllysate.

Immunocytochemistry:useat10ug/ml.

ImmunocytochemicalstainingofTACEinHeLacellswithTACEantibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.