Anti-TRAIL(CT)Antibody
CatalogNo.:2430
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.
ApoptosisisinducedbycertaincytokinesincludingTNFandFasligandoftheTNFfamilythroughtheirdeathdomain-containingreceptors,TNFR1andFas.AnovelmemberoftheTNFfamilyhasbeenidentifiedanddesignatedTRAIL(TNF-relatedapoptosis-inducingligand)andApo-2L(Apo-2ligand).TRAILisatypeIImembraneproteinandexpressedinavarietyofhumantissues.Twonoveldeathdomain-containingreceptors,DR4andDR5,havebeenidentifiedasthereceptorforTRAIL.LikeTNFandFasligand,TRAILinducesapoptosisandNF-Bactivationinmanytissuesandcelltypes.
Antigen:Syntheticpeptidecorrespondingtoaa261-277ofhumanTRAIL(accessionno.NP_003801).
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanTRAIL.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofTRAILinHeLacelllysateexpressing10,2.5,or1ngofrecombinantextracellulardomainofTRAILwithTRAILantibodyat1μg/ml.Themwoffull-lengthhumanTRAILis32kDa.
Positivecontrol:HeLacelllysate.
Immunocytochemistry:useat20ug/ml.
ImmunohistochemistryofTRAILinhumanbrainwithTRAILantibodyat20μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
