ORDERINGINFORMATION

Catalognos.:56354-50(50ug)56354-200(200ug)

Format:ProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide.

BACKGROUND

Ubiquitinisahighly-conserved,76-aminoacidpolypeptideexpressedinalleukaryoticcells.Ubiquitinationofproteinsfacilitatestargetingofcytosolicandnuclearproteinsfordegradationbyproteasomes.Thisessentialcellularprocessinvolvesamulti-enzymecascadeofubiquitin-activatingenzymes(E1s),ubiquitin-conjugatingenzymes(E2sorUbcs),andubiquitin-proteinligases(E3s).UbiquitinationisalsoinvolvedininternalizationanddegradationofplasmamembraneproteinssuchasTcellreceptorsubunitswhilestillassociatedwithmembranesoftheendoplasmicreticulum.

SPECIFICATIONSUMMARY

Antigen:NativebovineubiquitinconjugatedtoKLH.

Accessionno.:NP_776558

GeneID:281370

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizesfreeubiquitinaswellasubiquinatedproteinsinimmunoblots.Itrecognizeshuman,monkey,mouse,rat,hamster,rabbit,Guineapig,bovine,porcine,canine,sheep,chicken,Xenopus,yeast,Drosophila,andfishubiquitin.

APPLICATIONS

Immunoblotting:Useat1-5ug/ml.Abandof

~10kDaisdetected.

Immunohistochemistry:useat1-10ug/ml.

Detectionofubiquitininhumanbraincortex,senileplaque.

Detectionofubiquitininmousebrainlysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20°C.Storeinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.