CatalogNo.:2431

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

Anovelserine/threoninekinasethatmediatesapoptosishasbeenidentifiedanddesignatedZIPkinase.ZIPkinasecontainsanN-terminalkinasedomainandaC-terminalleucinezipperstructureandbindstoATF4,amemberofATF/CREBfamily.

ZIPkinasehashighsequencehomologytoDAPkinase(death-associatedproteinkinase),amediatorofapoptosisinducedbygammainterferon.OverexpressionofZIPkinaseinducesapoptosis.ZIPandDAPkinasesrepresentanovelkinasefamilywhichmediatesapoptosisthroughtheircatalyticactivities.MessengerRNAforZIPkinaseiswidelyexpressedinavarietyofhumantissues.

Antigen:Peptidecorrespondingtoaa279-298ofhumanZIPkinase(accessionno.

BAA81746).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

Thisantibodyrecognizesfull-lengthhuman,mouse,andratZIPkinase(52kDa).

Immunoblotting:useat1ug/ml.

WesternblotanalysisofZIPkinaseinHeLa(H)andJurkat(J)celllysateswithZIPkinaseantibodyat1g/ml.

Positivecontrol:HeLacelllysate.

Immunocytochemistry:useat10ug/ml.

ImmunocytochemicalstainingofZIPkinaseinJurkatcellswithZIPkinaseantibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.