productdatasheet

BacillusanthracisLethalFactorAntigenMonoclonalAntibody

CatalogNos.:18729(105)and18730

(106).

Size:200ugpurifiedantibodyinPBS,pH7.4.

AnthraxtoxinproducedbyBacillusanthracisiscomposedofthreeproteins:ProtectiveAntigen(PA),EdemaFactor(EF),andLethalFactor(LF).Toxicityrequirestheassemblyofallthreeoftheseproteins,andthisprocessisinitiatedwhenPAbindstothecellsurfaceATR(anthraxtoxinreceptor).

Afterbinding,PAisenzymaticalycleavedtoyielda63kDfragmentwhichthenbindsEFandLF,andthecomplexisinternalized.LFisaproteaseandcleavesenzymesoftheMAPKK(mitogen-activatedproteinkinasekinase)family.

Antigen:PurifiedB.anthracisLethalFactor(82kD).

HostSpecies:MouseAntibodyClass:IgG1Preservative:0.1%NaN3

TheseantibodiesrecognizedifferentepitopesofB.anthracisLF.Theydonotcross-reactwithB.anthracisPA,EF,orspore/vegetativesurfaceantigens.

Immunoblotting:useat5ug/ml.SandwichELISA:usecoatingantibody(18730)at5ug/ml;usedetectantibody(18729)at1ug/ml.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Theseantibodiesarestableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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