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BacillusanthracisProtectiveAntigenMonoclonalAntibody

CatalogNos.:18720(C3),18721

(101),18722(102),18723(103),18724

(104),18725(105),and18726(106).

Size:200ugpurifiedantibodyinPBS,pH7.4.

AnthraxtoxinproducedbyBacillusanthracisiscomposedofthreeproteins:ProtectiveAntigen(PA),EdemaFactor(EF),andLethalFactor(LF).Toxicityrequirestheassemblyofallthreeoftheseproteins,andthisprocessisinitiatedwhenfull-lengthPAbindstothecellsurfaceATR(anthraxtoxinreceptor).Afterbinding,PAisenzymaticallycleavedtoyielda63kDfragmentwhichthenbindsEFandLF,andthecomplexisinternalized.

Antigen:PurifiedB.anthracis

ProtectiveAntigen(83kD).

HostSpecies:Mouse

AntibodyClass:IgG1=18720,18725,18726;IgG2b=18721,18722,18723,

18724

Preservative:0.1%NaN3

Theseantibodiesrecognizedifferentepitopesofthe83kDand63kDformsofB.anthracisPA.Theydonotcross-reactwithB.anthracisEF,LF,orspore/vegetativesurfaceantigens.

Immunoblotting:use18721,18723,

18725,or18726at5ug/ml.

SandwichELISA:usecoatingantibody(18720or18725)at5ug/ml;usedetect

antibody(18722,18723,or18726)at1ug/ml.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Theseantibodiesarestableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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