FrancisellatularensisLPSMonoclonalAntibodies

Francisellatularensisisasmall,nonmotile,aerobic,gram-negativecoccobacillusandtheetiologicagentoftularemia.Ithasathinlipopolysaccharide-containingenvelopeandisaresilientnon-spore-formingbacteriumthatcansurviveforseveralweeksatlowtemperaturesinwater,soil,hay,anddecayinganimalcarcasses.Lipopolysaccharide(LPS)isamajorspecies-specificantigenofFr.tularensis.

Antigen:#18741(T14)wasderivedfrommiceimmunizedwithpooledwater-solubleantigensfromdisintegratedmicrobialmassofFr.tularensisvaccinestrain.

#18742(FB11)wasderivedfrommiceimmunizedwithwholeFr.tularensis.HostSpecies:Mouse

#18742(FB11)=IgG2a

Theseantibodiesarestableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

TheseantibodiesspecificallyrecognizeFr.tularensisLPS.Theydonotcross-reactwithFr.novicida,B.abortus,B.suis,B.melitensis,B.ovis,B.neotomae,

Y.pestis,Y.pseudotuberculosis,Y.enterocolitica,S.typhimurium,

V.cholera,orE.coli.

Theepitopefor#18742(FB11)islocatedontheO-antigenpolysaccharidechainofFr.tularensisLPSwhichconsistsoftetrasaccharidefragments.

TetrasaccharideD-GalpNAcAN-(1-4)-alpha-D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFmandtrisaccharideD-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFmcompetewithLPSofFr.tularensisinELISAforbindingMAb#18742(FB11).

Theepitopefor#18741(T14)hasnotbeenidentified.

ELISA:useat1-5ug/mlImmunofluorescence:useat5-10ug/mlThesearerecommendedconcen-trations;endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.