productdatasheet
CatalogNo.:3302
Size:100ugor200ugProteinG-purifiedantibodyinPBS,pH7.4
Antigen:GSTfusionproteinexpressedin
E.colicorrespondingtoaa762-1315offull-lengthBRCA1.
HostSpecies:MouseAntibodyClass:IgG1/κStABIlizers:None
Preservatives:None.Availableonrequest.
Thisantibodyrecognizesfull-lengthBRCA1,a220kDnuclearphosphoprotein,anddoesnotrecognizetheΔexon11splicevariant.Inahighproportionofbreastandovariancancercelllines,BRCA1aberrantlymislocatestothecytoplasm.ItsusefulnesstomonitorfunctionalinactivationofBRCA1insporADIcbreastcancersisunderactiveinvestigation.
APPLICATIONSImmunoblotting:useat1-10ug/ml.Immunoprecipitation:useat1-10ug/ml.Immunohistochemistry:useat1-10ug/ml.Positivecontrols:Anynormalhumantissue,especiallythosecontainingrapidlyproliferatingcells,suchaslymphoidgerminalcenters.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.
Chen,Yetal.(1995)Science270:789-791;Chen,C-Fetal.(1995)JBiolChem271:32863-32868;Chen,Yetal.(1996)
CancerRes56:3168-3172.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures
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