CatalogNo.:34032

Size:100ulantiserum

Antigen:NH2terminalcysteine-peptidecorrespondingtoaa2019-2032inthecarboxylterminalregionofhumanch-TOGcoupledtoKLH:

NH2-CDLKKRLERIKSSRK-COOH.

HostSpecies:Rabbit

AntibodyClass:PolyclonalPreservatives:None.Availableonrequest.

Colonicandhepatictumorover-expressedprotein(ch-TOG)belongstotheTOG/XMAP215/Dis1evolutionarilyancientfamilyofmicrotubule-associatedproteinswithisoformsfoundinallmajorkingdomsofeukaryotes.Thisproteinisrequiredfortheassemblyandfunctionofthemeioticormitoticspindlesduringcelldivisionandislocalizedtomicrotubule-organizingcenters(MTOCs)duringinterphaseortothespindlepolesandcentrosomesduringmitosisorcytokinesis.

Thisantibodyrecognizeshumanandmousech-TOG.ABLASTsearchdemonstrated100%identitytothepeptideimmunogeninhuman,mouse,rat,chimpanzee,dog,cow,chicken,opossum,andfrog.

Immunoblotting:useat1:5,000-1:10,000dilution.Abandofapprox.215kDaisdetected.

Immunoprecipitation:useat1:5,000-1:10,000dilution.

WCLNII

IP

WesternblotonhumanHelawholecelllysate(WCL)andch-TOGimmunoprecipitated(I)frommouseembryonicfibroblastwholecelllysate.Thech-TOGantibodydidnotreactwiththemouseembryonicfibroblastwholecelllysatewhennon-immunesera(NI)wasusedintheimmunoprecipitationreaction.Proteinswereresolvedandtransferredfroma9%SDS-PAGE.

Immunofluorescence:useat1:5,000-1:10,000dilution.

Immunofluorescentimages(100X)ofamousefibroblastcelllinelabeledwithch-TOG(red),microtubules(green)demonstratingch-TOGlocalizeswithmicrotubules(yellow).

Thesearerecommendedconcentra-tions.Usershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.