productdatasheet
CatalogNo.:56220
Size:100ugProteinG-purifiedantibodyinPBS,pH7.4
Antigen:RecombinanthumanDNALigaseIII(aa1-862)purifiedfrombaculovirusinfectedSf9cells.
HostSpecies:MouseAntibodyClass:IgG1/κStABIlizers:None
Preservatives:None.Availableonrequest.
ThisantibodyrecognizeshumanDNALigaseIII,oneofthefourligaseproteinsfoundinmammaliancellsthatarerequiredforthejoiningofDNAstrandbreaksthatoccurduringDNAreplication,repair,andrecombination.DNALigaseIIIisfoundasanα-form(100kD)insomatictissuesandasaβ-form(97kD)intestis.
APPLICATIONSImmunoblotting:useat1-5ug/ml.Positivecontrols:MCF-7andT24cellsorrecombinantprotein.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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