CatalogNo.:3335

Size:100ugProteinG-purifiedantibodyinPBS,pH7.4,1mg/ml.

Antigen:6-Hisfusionproteincontainingtheregionencodingaa1-190ofhumanestrogenreceptor-α

(ER-α)expressedinE.coli.HostSpecies:MouseAntibodyClass:IgG1/κStABIlizers:None

Preservatives:None.Availableonrequest.

ThisantibodyrecognizeshumanandrabbitER-α,a65kDproteinandamemberofthesteroidfamilyofnuclearreceptors.ER-αisaligand-activatedtranscriptionfactorthat,whenboundtoestrogen,inducesaconformationalchangethatallowsdimerizationandbindingtoestrogenresponseelementsequences.WhenboundtoDNA,ER-αcanpositivelyornegativelyregulategenetranscription.

Immunoblotting:useat1-5ug/ml.

30ugofMCF7wholecelllysate,7.5%SDS-PAGE,#3335at2ug/ml.

Immunohistochemistry:frozensections,useat1-10ug/ml.

FACS:useat1-10ug/ml.Immunoprecipitation:useat1-5ug/ml.Positivecontrols:MCF-7cellsandrecombinantprotein.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat≤-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.