ORDERINGINFORMATION
CatalogNo.:34038(cloneFIH162C)Format:100uginPBS,pH7.4,0.09%sodiumazide,50%glycerol.PurifiedbyProteinGaffinitychromatography.BACKGROUND
FIH,FactorInhibitingHIF-1(hypoxia-inducIBLefactor),isanasparaginylhydroxylase.FIH,inconjunctionwithVHL,repressesHIF-1transcriptionalactivitybydisruptingtheinteractionofHIF-1withthetranscriptionalcoactivatorsCBP/p300,andbyrecruitinghistonedeacetylases.FIHactivityisinhibitedduringhypoxia.RecentstudiesshowthatlownuclearexpressionofFIHisaprognosticindicatorforpooroverallsurvivalincasesofclearcellrenalcellcarcinoma.
SPECIFICATIONSUMMARY
Antigen:Recombinantfull-lengthhumanFIHexpressedinE.coli.
Accessionno.:Q9NWT6,NP_060372.2HostSpecies:MouseAntibodyClass:IgG1Specificity:ThisantibodyrecognizeshumanFIH.APPLICATIONS
Immunoblotting:useat10ug/ml.Abandof
~45kDaisdetected.
Immunofluorescence:useat10ug/ml.
DetectionofFIHinHeLacelllysate.
DetectionofFIHinformaldehyde-fixedHeLacells.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticprocedures.
