ORDERINGINFORMATION

CatalogNo.:3338

CloneNo.:7H3

Format:100ugProteinG-purifiedantibodyinPBS,pH7.4.

BACKGROUND

FKHR(ForKHeadinRhaBDomyosarcoma),a70kDaprotein,isamemberoftheforkheadfamilyoftranscriptionfactors.Membersofthisfamilyareclassifiedbytheirdegreeofhomologyinthe100-120aaregionthatisnecessaryforDNAbindingandbinding-sitespecificity.TheFKHRsubfamilydiffersfromothermembersoftheforkheadfamilyinthatallmemberscontainaninsertionof5aminoacidsnexttothehelix3inthemiddleoftheforkheaddomainandlacktheconservedaminoterminalKPPYsequencefoundinmostforkheadgenes.IthasbeenshownthatmembersoftheFKHRgenefamilyarephosphorylatedbyAktwhichregulatesitscellularlocalizationpromotingcellsurvival.

SPECIFICATIONSUMMARY

Antigen:GSTfusionproteincontainingtheregionencodingaa242-655ofhumanFKHRexpressedinE.coli.

HostSpecies:Mouse

AntibodySubtype:IgG1

Specificity:ThisantibodyrecognizeshumanFKHR/FOX01.

APPLICATIONS

Immunoblotting:useat0.1-2.0ug/ml.Abandof70kDaisdetected.

IHC/ICC:

ImmunofluorescentdetectionofFKHR/FOX01inparaformaldehyde-fixedHeLacellswith#3338at10ug/ml.

Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DetectionofFKHR/FOX01inparaffin-embeddedcoloncarcinomawith#3338at20ug/ml.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oCorbelow.Storeundilutedproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.