productdatasheet
CatalogNo.:34029
Size:100ugProteinG-purifiedantibodyinPBS,pH7.4.
SPECIFICATIONSUMMARYAntigen:Recombinantfull-lengthhumanglypican-1.
Cloneno.:9E9
HostSpecies:MouseAntibodyClass:IgG1StABIlizers:None
Preservatives:None.Availableonrequest.
BACKGROUNDGlypican-1isawidelyexpressed,GPIanchored,cellsurfaceproteoglycanthathasbeenreportedtobeimportantincellularadhesionandmigration.
Glypican-1alsofunctionsasanaccessorymoleculeforsignalingreceptors.Glypican-1isoverexpressedinhumangliomasandpancreaticductalcellcarcinomas.Expressionofglypican-1isassociatedwiththeaggressivebehavioroftumorcellsinvitroandinvivo.
SPECIFICITYThisantibodyrecognizesnativeandrecombinanthumanandmouseglypican-1.
APPLICATIONSELISA:at1-5ug/mlwilldetectrecombinantglypican-1onthesolidphaseat200ng/well.
APPLICATIONS(continued)Immunoblotting:at1-10ug/ml,abandofapprox.64kDaisdetectedinnormalmousebrain.Immunofluorescence:at1-10ug/ml,positivestainingofhumanpancreaticcarcinomacelllineCapan-1.
Thesearerecommendedconcen-trations.Usershoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONSDiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITYThisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
