
Anti-HGALMonoclonalAntibody
Thehumangerminal-center-associatedlymphoma(HGAL)genewasoriginallyidentifiedfromanexpressedsequencetagthatwasassociatedwithimprovedsurvivalinpatientswithdiffuselargeB-celllymphoma(DLBCL).CloningandcharacterizationofHGALindicatedthatitisinducedbyIL-4.
HGALisacytoplasmicproteinandhasbeendemonstratedinfollicularlymphoma,Burkittlymphoma,lymphocyte-predominantHodgkinlymphoma,andasubsetofDLBCL.HGALexpressionisanimportantpredictorofoverallsurvivalinpatientswithgerminalcenter-derivedtumors.HGALproteinisalsofoundingerminalcentersofnormaltonsilsandlymphnodes.
ThisantibodyrecognizeshumanHGAL.
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thaw
cycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
APPLICATIONSImmunohistochemistry:useat1-10ug/mlonparaffin-embeddedtissueswithheat-inducedantigenretrievalincitratebuffer(10mM,pH6.0,10minutes).
Positivestainingoflymphnodefollicularandinterfollicularregionswith#34044.
PositivestainingofHGALwith#34044inclassicHodgkinlymphoma.
Immunoblotting:useat1-10ug/ml
HGALmRNAandproteinexpressioninlysatesofindicatedcelllines.ActinservedasaloADIngcontrol.Thesearerecommendedconcentrations.
Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
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