ORDERINGINFORMATION
CatalogNo.:34039(cloneESEE122)Format:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.BACKGROUND
Hypoxia-inducIBLefactor1(HIF-1)isaheterodimerictranscriptionfactorthatregulatesthetranscriptionofabroadrangeofgenesthatfacilitateresponsestohypoxiaincludinggenesthatregulateangiogenesis,erythropoiesis,cellcycle,metabolism,andapoptosis.HIF-1iscomprisedoftwosubunits,HIF-1αandHIF-1β.ThewidelyexpressedHIF-1αisdegradedrapidlyinnormoxiccellsbytheubiquitin/proteasomalpathway.HIF-1αisprolinehydroxylatedleADIngtoaconformationalchangethatpromotesbindingtothevonHippelLindauprotein(VLH)-E3ligasecomplex;ubiquitinationandproteasomaldegradationfollows.
SPECIFICATIONSUMMARY
Antigen:Recombinantproteincorrespondingtoaa329-530ofhumanHIF-1α.Accessionno.:Q61221,NP_034561.2HostSpecies:MouseAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,rat,andbovineHIF-1α.APPLICATIONS
Immunoblotting:useat2ug/ml.Predictedmolecularweight~116kDa.
Thisisarecommendedconcentration.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DetectionofHIF-1αinHeLacelllysate.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
