ORDERINGINFORMATION
CatalogNo.:34040(cloneEP190b)Format:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.BACKGROUND
Membersofthehypoxia-inducIBLefactor(HIF)familyoftranscriptionfactorsregulatecellularresponsestohypoxia.HIF-2αisinvolvedincatecholaminehomeostasis,vascularremodeling,physiologicalangiogenesis,andADIpogenesis.Itisoverexpressedinmanycanceroustissues.Studiessuggestthatinnon-smallcelllungcancer,HIF-2αisapromoteroftumorgrowthandprogressioninasolidtumor.
SPECIFICATIONSUMMARY
Antigen:Fusionproteincorrespondingtoaa535-631ofhumanHIF-2α.Accessionno.:Q99814,NP_001421.4HostSpecies:MouseAntibodyClass:IgG1Specificity:ThisantibodyrecognizeshumanHIF-2α.APPLICATIONS
Immunoblotting:useat1ug/ml.Predictedmolecularweight~96kDa.
Immunofluorescence:useat10ug/ml.
DetectionofHIF-2αinCoCl-treatedHeLacelllysate.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DetectionofHIF-2αinformaldehyde-fixedHeLacells.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
