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CatalogNo.:3339

Size:100ulProteinG-purifiedantibodyat1mg/mlinPBS,pH7.4.

Antigen:Recombinanthumanp53expressedinE.coli.

HostSpecies:MouseAntibodyClass:IgG2a/κStABIlizers:None

Preservatives:None.Availableonrequest.

Thisantibodyrecognizesanepitopewithinaa20-25attheN-terminusofhumanp53.p53isanuclearproteinandtumorsuppressorthatismutatedinawidevarietyofcancers.ItmediatescellcyclearrestinresponsetoDNAdamage,makingitimportantinthemaintenanceofgenomicstability.

Immunoblotting,immunoprecipitation,immunohistochemistry,andELISA:useat1-5ug/ml.

Positivecontrol:HBL100cells.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.

REFERENCE

VojtesekB.1992.JImmunolMeth151:237-244.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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