CatalogNo.:3340

Size:100ugProteinG-purifiedantibodyinPBS,pH7.4

Antigen:Gel-purifiedp53containingaa14-389ofhumanp53.

HostSpecies:Mouse

AntibodyClass:IgG1

Preservatives:None.Availableonrequest.

Thisantibodyrecognizeshumanp53,atumorsuppressorproteinthatismutatedinawidevarietyofcancers.p53isanuclearproteinthatmediatescellcyclearrestinresponsetoDNAdamagewhichisimportantinmaintaininggenomicstABIlity.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

IggoRetal.1990Lancet335:675-679.

GannonJVetal.1990EMBO9:1595-1602.

SaidJWetal.1992AmerJPath141:1343-1348.

BartokJetal.1991Oncogene6:1699-1703.

ELISA,FACS,IHC(frozenandparaffin),Immunoprecipitation,Immunoblotting.

IHC:useat2-4ug/ml.Extensivewashingmaybenecessary.Thisproduct

doesnotrequireproteindigestionormicrowaveantigenretrievalpriortostaining.

#3340onparaffin-embeddedHBL435xenograft.

IP:useat10ug/mglysate.ThisantibodyreactswithonlyMUTANTp53proteinundernon-denaturingconditions.

Immunoblotting:useat0.5-1.0mg/ml.Abandof53kDaisdetected.

#3340on30ugof293T(A)andA431(B)celllysates.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures