productdatasheet
CatalogNo.:56253(clone1320)Size:100ugProteinG-purifiedantibodyat1mg/mlinPBS,pH7.4.
Antigen:Recombinantproteincorrespondingtothep37subunitofhumanReplicationFactorCexpressedinE.coli.
HostSpecies:MouseAntibodyClass:IgG2bStABIlizers:None
Preservatives:None.Availableonrequest.
Thisantibodyrecognizesthep37subunitofhumanandyeast(S.cerevisiae)ReplicationFactorC(RFC).RFCisaheteropentamericproteinconsistingofp140,p40,p38,p37,andp36subunits,RFCparticipatesintargetingDNApolymeraseδprocessivityfactor(PCNA)ontoaprimedDNAtemplateduringDNAsynthesisandrepair.RFChasalsobeenassociatedwithDNArepairinconjunctionwithaPCNA-likeheterotrimerofHus1,Rad1,andRad9.
APPLICATIONSImmunoblotting:useat1:2,000dilution.Abandof37kDisdetected.
Positivecontrols:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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