Catalogno.:34045Antigen:Bases2535-2835ofmouseROR2Cloneno.:ROR22535-2835fusedin-frametoMBPandexpressedinE.coli.Size:100uginPBS,pH7.4.HostSpecies:Mouse

PurifiedbyProteinGaffinitychromatography.AntibodyClass:IgG1

Receptortyrosinekinases(RTKs)arecellsurfacereceptorsthatregulatenormalcellularprocessesthroughligand-controlledtyrosinekinaseactivity.ROR2isamembrane-boundRTKthatisactivatedbyWntsignalingduringnormalboneandcartilagedevelopment.Recently,ROR2hasbeenshowntohavepro-tumOrigeniceffectsinosteosarcoma,melanoma,andrenalcellcarcinomacelllines.ROR2levelshavealsobeendescribedinsoft-tissuesarcomas;specifically,invitrostudiesrevealedthatinvasiveABIlitiesofleiomyosarcoma(LMS)andgastrointestinalstromaltumor(GIST)areaffectedbyROR2expression,andsuppressionofROR2reducesinvivotumormassinaxenotransplantationmodelofLMS.

Immunoblotting:useat1-10ug/ml.Abandof~105kDaisdetected.

Immunohistochemistry:useat1-10ug/mlonparaformaldehyde-fixed,paraffin-embeddedsectionsfollowingantigenretrieval(microwave12minincitratebuffer,pH6.0).

Thisantibodyisstableforatleastone(1)yearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

DetectionofROR2inLMSandGIST.Sampleswerescored2(strongstaining),1(weakstaining),or0(absenceofstaining).SeereferenceEdrisB.etal.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.

PRODUCTREFERENCESForinvitroinvestigationaluseonly.NotMikelsAetal.2009JBiolChem284:30167-30176.foruseindiagnosticortherapeuticWrightTMetal.2009Oncogene28:2513-2523.applications.

EdrisBetal.2012JPathol227:223-233