productdatasheet
CatalogNo.:56216
Size:100ugProteinG-purifiedantibodyinPBS,pH7.4
Antigen:N-terminally6x-Histaggedfusionproteincorrespondingtofull-lengthhumanRPA32expressedin
E.coli.
HostSpecies:MouseAntibodyClass:IgG2a/κStABIlizers:None
Preservatives:None.Availableonrequest.
ThisantibodyrecognizeshumanandmouseRPA32.RPA(ReplicationProtein
A)isasingle-strandedDNAbindingprotein.HumanRPAisaheterotrimericproteincontainingsubunitsof70,32,and14kD.ThisproteincomplexishighlyconservedineukaryotesandisessentialinDNAreplication,homologousrecombination,andnucleotideexcisionrepair.RPA32isphosphorylatedduringtheS-phaseofthecellcycle,inresponsetoDNAdamage,andduringapoptosisofJurkatT-lymphocytes.
APPLICATIONSImmunoblotting,immunoprecipitation:useat0.2-1ug/ml.
Positivecontrol:HeLa,Raji,and3T3cellsandrecombinantprotein.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.
Iftode,Cetal.1999.CritRevBiochemMolBiol34:141-180;Bochkarev,Aetal.1999.
EMBO18:4498-4504;Dong,Jetal.1999.
BiochemJ337:311-317;Sibenaller,ZAetal.1998.Biochemistry37:12496-12506;Stigger,Eetal.1998.JBiolChem273:9337-9343;Bochkareva,Eetal.1998.JBiolChem273:3932-3936.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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