productdatasheet
CatalogNo.:34021(cloneG8)Size:100ugProteinG-purifiedantibodyinPBS,pH7.4
Theskioncogenefamily(skiandsno)encodestranscriptionalregulatorsthatcanaffectoncogenictransformationandcellulardifferenti-ation.skiandsnofunctionasco-activatorsorco-repressors,modulatingtranscriptionbyinter-actingwithtranscriptionfactorcomplexes.skiinteractswithmembersoftheNuclearFactorI(NFI)family,theretinoicacidreceptor,andthenuclearhormoneco-regulatorsNCoRandNCoA62/skip.
PRODUCT-SPECIFICREFERENCES
Tarapore,Petal.(1997)NucleicAcidsRes25:3895-3903.
Colmenares,Cetal.(1991)MolecularandCellularBIOLOGy11:1167-70.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICATIONSUMMARYImmunogen:Recombinantv-skiHostSpecies:MouseAntibodyClass:IgG1StABIlizers:None
Preservatives:None.Availableonrequest.
Thisantibodyrecognizesskiproteinofhuman,mouse,rat,chicken,andXenopus.
Immunoblotting:useat1-5ug/ml.Predictedmolecularweightofv-skiis55kDa;full-lengthskirangesfrom~95kDaupto~115kDaforhyperphosphorylatedski.
Immunocytochemistry:useat1-10ug/ml.skiisanuclearprotein.
Immunoprecipitation:useat1-5ug/ml.Positivecontrols:Recombinantcelllinesexpressingskiproteins.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.
QEDBioscience,Inc.10919TechnologyPlace,SuiteC
SanDiego,CA92127
TollFree800.929.2114
Phone858.675.2405
Fax858.592.1509
info@qedbio.com
Visitourwebsiteforadditionalproductinformationandtoorderonline.
qedbio.com
