productdatasheet

CatalogNo.:34003(3B3.E11)

Size:200ugProteinG-purifiedantibodyinPBS,pH7.4.

Thymidinekinase(TK)belongstoagroupofenzymessuchasdihydrofolatereductase,thymidylatesynthase,andDNApolymerasethatareinvolvedinDNAsynthesisandprecursorproduction.Highlevelsoftheseenzymesarepresentinproliferatingcells,andlowlevelsarefoundinrestingcells,duetomultipleregulatorymechanismsthatensureexclusiveexpressionoftheseenzymesinreplicatingcells.TKisresponsIBLeforcatalyzingthephosphorylationofthymidine,whichfunctionsasapartofthepyrimidinesalvagepathwayinvolvedinDNAsynthesis.TheactivitiesofenzymessuchasTKareessentialfortheactivationofseveralchemotherapeuticallyimportantnucleosideanaloguesthatareadmiNISTeredasprodrugs.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Antigen:5’-His-taggedrecombinanthumanthymidinekinase.

HostSpecies:MouseAntibodyClass:IgG1StABIlizers:None

Preservatives:None.Availableonrequest.

Thisantibodyspecificallyrecognizeshumanthymidinekinase.

Immunoblotting:antibodyat10ug/mlwillrecognizeTKin5,10,or20uglysatesofcellsthatexpressTK.ELISA:useantibodyat2-5ug/ml(optimizedforTKonsolidphaseat10ug/ml).

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.