productdatasheet

CatalogNo.:34001(2D8.D11)and34002(3A7.A11).

Size:200ugProteinG-purifiedantibodyinPBS,pH7.4.

Thymidylatesynthase(TS)isanintracellularenzymecriticalfordenovosynthesisofDNA.Incancer,expressionofTSisoftenelevatedandbecomesfurtherelevatedasaresultoftreatmentwiththemostcommonlyusedchemotherapeutic,5-fluorouracil(5-FU).Resistanceorlackofresponseto5-FUisattributedtotheelevationofTSactivity.OverexpressionofTShasbeendemonstratedinmanytypesoftumorsincludingbreast,colon,gastricandmelanomaandisusuallypredictiveofshortenedsurvivaltime.

Antigen:Recombinanthumanthymidylatesynthaseproducedin

E.coli.

HostSpecies:MouseAntibodyClass:IgG1StABIlizers:None

Preservatives:None.Availableonrequest.

TheseantibodiesspecificallyrecognizehumanTS(36kDa).Theydonotcross-reactwithratTS.

Immunohistochemistry:useat5-10ug/mlonfrozensections(reactivityonparaffin-embeddedsectionshasnotbeendetermined).

Immunoblotting:useat5-10ug/ml.ELISA:useat2-5ug/ml(optimizedforTSonsolidphaseat5ug/ml).

Immunoprecipitation:use#34002onlyat5-10ug/ml.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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